膜科学与技术

Position：Home >> Abstract

Study on optimizing the preparation conditions and characterizing of novel nylon affinity membranes

Authors： NIE Huali1², Chen Tianxiang², ZHU Limin²

Units： 1. Key Laboratory of Science & Technology of Eco-Textil, Ministry of Education, Donghua University Shanghai 201620, China; 2. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China

KeyWords： Affinity membrane chromatography；chitosan；papain；cystatin

ClassificationCode：TQ028.8

year,volume(issue):pagination： 2011,31(2):74-77

Abstract：

Nylon membranes are first hydrolyed with dilute HCl and then treated with chitosan before being used as the affinity carrier. Papain as a ligand has been immobilized on the actived membranes with glutaraldehyde as crosslinking agent. The factors involving with the activity of immobilized papain, such as concentration of glutaraldehyde, pH, temperature, reaction time, and the amount of added papain have been studied. The results show that the optimum conditions for papain immobilization are as follows: pH 9.0, 0.5% glutaraldehyde solution, the concentration of added papain is 10 mg/mL, the reaction time is 6 h at 45℃. The nylon membrane prepared is then used to purify cystatin from potato juice, and showed that they are high efficiency affinity membranes base for cystatin separation.

Funds：

国家自然科学基金（50773009）；教育部高校博士点新教师基金（20090075120001）；中央高校基本科研业务费专项基金

AuthorIntro：

聂华丽（1978-）, 女，上海市人，博士，讲师，从事蛋白质分离与纯化研究，电话：021-67792659，E-Mail：niehuali2004@126.com

Reference：

[1] Fossum K, Whitaker J R. Ficin and papain inhibitor from chicken egg white[J]. Arch Biochem Biophys, 1968, 125(1):367－375.
[2] North M J, Mottram J C, Coombs G H. Cysteine proteinases of parasitic protozoa[J]. Parasitology Today, 1990, 6(8)：270－275.
[3] Zeng Z K, Bao J K, Zhou H, et al. The molecular modification of cysteine proteinase inhibitor from wheat seed and its activity[J]. Scientia Agriculture Sinica, 1997, 30(1):38－43.
[4] Beeskow T C, Kusharyoto W, Anspach F B, et al. Surface modification of microporous polyamide membranes with hydroxyethyl cellulose and their application as affinity membranes[J]. J Chromatogr A,1995, 715(1):49－65.
[5] Lei H, Wang W, Chen L L, et al. The preparation and catalytically active characterization of papain immobilized on magnetic composite microspheres[J]. Enzyme Microb Tech, 2004, 35(1):15－21.
[6] 黄宜兵, 吴晓霞, 吴胜男, 等. 以卵清蛋白为载体的固定化木瓜蛋白酶的制备及其性质[J]. 吉林大学学报(理学版), 2004, 42(4):616-621.
[7] 贺枫,卓仁禧, 刘立建, 等. 固定化木瓜蛋白酶的制备和性质研究[J]. 高分子学报, 2000, 10(5):637－640.
[8] Wei S, Zhang F B, Zhang G L. Adsorption of bilirubin with polylysine carrying chitosan-coated nylon affinity membranes[J]. J Chromatogr B, 2005, 819(2):301－306.
[9] 苏赛男，聂华丽，朱利民，陈天翔. 尼龙亲和膜色谱法纯化半胱氨酸蛋白酶抑制剂[J]. 分析化学, 2008, 2(36): 231－234.

Service：

【Download】【Collect】

《膜科学与技术》编辑部 Address: Bluestar building, 19 east beisanhuan road, chaoyang district, Beijing; 100029 Postal code; Telephone：010-80492417/010-80485372; Fax：010-80485372 ; Email：mkxyjs@163.com