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Endotoxin removal from human plasma through PVDF affinity membrane containing Ser ligand

Authors： Kun Xu1, Man Huang3, Liguang Wu2, Lin Zhang1, Huanlin Chen1

Units： 1. Department of Chemical & Biochemical Engineering, Zhejiang University, Engineering Research Center of Membrane and Water Treatment Technology, MOE, Hangzhou, 310027, China2. College of Environ. Sci. & Eng., Zhejiang Gongshang University, Hangzhou, 310035, China;3. Sir Run Run Shaw Hospital, Zhejiang University, 310016, China

KeyWords： endotoxin; affinity membrane; PVDF; Ser; human plasma

ClassificationCode：TQ028.8

year,volume(issue):pagination： 2011,31(2):28-34

Abstract：

A new affinity membrane, PVDF hollow fiber membrane with L-serine (Ser) ligand was prepared (with 1,6-hexanediamine as the space arm) for endotoxin removal from human plasma. FTIR and X-ray photoelectron spectroscopy (XPS) were used to analyse the surface of membranes. The effects of operation parameters，such as pH value and ionic strength，on the endotoxin adsorption capacity of the affinity membranes were investigated, so as to the affinity adsorption kinetics. Then, self-made affinity membrane module was used to remove endotoxin form human plasma., and the endotoxin adsorption capability is 0.029EU/cm2. For the human plasma sample with endotoxin concentration of 0.42EU/ml, the clearance efficiency of self-made module could reach 43.8% with little effect on other biochemical indicators.

Funds：

浙江省科技厅科技计划项目（2007C33008）；中央高校基本科研业务费专项资金资助（教育部膜与水处理技术工程研究中心, 浙江大学，KYJD09011）

AuthorIntro：

徐堃（1983-），男，山东烟台人，硕士研究生，主要从事亲和膜脱除内毒素研究. *通讯联系人

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