膜科学与技术

Position：Home >> Abstract

Characteristics and Immobilization Process of CS-Cu Enzymatic Membrane

Authors： Ge Zheng1, Cai Bangxiao1，2 *

Units： 1.Institute of Membrane Science and Engineering, College of Food and Biological Engineering, Zhejiang Gongshang University, Hangzhou 310035）

KeyWords： enzymatic membrane reactor; immobilized enzyme; chitosan-Cu complex; polyphenol oxidase; response surface

ClassificationCode：TQ028.8,R978.1

year,volume(issue):pagination： 2012,32(1):64-70

Abstract：

Enzymatic membrane reactor(EMR) with immobilization of polyphenol oxidase(PPO) is paid more attention due to its isolation and biochemical characteristics. In this paper, a enzymatic membrane immobilized PPO was prepared based on the chelation of PPO and Cu2+ in chitosan(CS)-Cu complex(CCC) with CS membrane as the carrier. The response surface methodology was applied to investigate the influence of three factors of copper ion sequestration, QCu2+, PPO concentration, CE, pH in immobilization process on the vitality of PPO immobilized in CCC. The relative activity of PPO in the solution and immobilized in the membrane was determined respectively. The characteristics of CS-Cu–PPO membrane were discussed primarily. The results show that the optimal conditions for immobilization of PPO into the CCC were temperature 40℃, pH 7.67, QCu2+ 30.07 mg/mL, CE 2.12 mg/mL. Michaelis constant and the relative activity of PPO immobilized in CCC were larger than that of the solution. Enzyme half-decline period was 30 days, and the relative activity of PPO after repeated use of eight times reduced to 79% for CS-Cu–PPO membrane. This work offers the significantly basis for development of EMR with special function.

Funds：

国家科技支撑计划项目(2007BAD62B05), 国家农业部行业科技开发专项(nyhyzx07-21)

AuthorIntro：

葛振(1985-), 男, 山东人, 食品科学硕士研究生，研究方向：新型分离膜及其应用工程. *通讯联系人: 蔡邦肖, 教授. 13958002662, caibangxiao@163.com

Reference：

[1] S. T. Bouwer, F. P. Cuperus, and J. T. P. Derksen. The performance of enzyme-membrane reactors with immobilized lipase[J]. Enzyme and Microbial Technology, 1997, 21(1):291-296.
[2] G. M. Rios, M. P. Belleville, D. Paolucci, et. al. Progress in enzymatic membrane reactors – a review[J]. Journal Membrane Science, 2004, 242(1-2):189-196.
[3] L. J. Kennedy, P. K. Selvi, A. Padmanabhan, et al. Immobilization of polyphenol oxidase onto
mesoporous activated carbons – isotherm and kinetic studies [J]. Chemosphere, 2007, 69 (2):
262-270.
[4] S. Kiralp, L.Toppare, Y.Yagci. Immobilization of polyphenol oxidase in conducting copolymers
and determination of phenolic compounds in wines with enzyme electrodes[J]. International
Journal of Biological Macromolecules, 2003, 33 (1-3): 37-41.
[5] I. Mielgo, C. Palma, J. M. Guisan, et al. Covalent immobilization of manganese peroxidases (MnP) from Phanerochaete chrysosporium and Bjerkandera sp. BOS55[J]. Enzyme and Microbial Technology, 2003, 32 (7): 769-775.
[6] T. Gumi, D. Paolucci-Jeanjean, B. Marie-Pierre, et al. Enzymatic membrane reactor involving a hybrid membrane in supercritical carbon dioxide[J]. Journal of Membrane Science, 2007, 297(1-2): 98-103.
[7] B. Krajewska. Application of chitin- and chitosan-based materials for enzyme immobilizations: a
review [J]. Enzyme and Microbial Technology, 2004, 2(35): 126-139.
[8] N. Duran, E. Esposito. Potential application of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review[J]. Applied Catalysis B: Environmental, 2000, 28 (2): 83-99.
[9] J. Z. Lu, X. F. Duan, Q. L. Wu, et al. Chelating e?ciency and thermal, mechanical and decay resistance performances of chitosan copper complex in wood–polymer composites[J]. Bioresource Technology, 2008, 99 (13 ) :5906-5914.
[10] 姜梅, 王善荣, 季勤. 壳聚糖膜固定化葡萄糖氧化酶的特性研究[J]. 食品科学, 2003, 24(4): 38-40.
[11] 黄意欢. 茶学实验技术 [M]. 北京:中国农业出版社, 1995: 213-214.
[12] 雷福厚. 壳聚糖铜固定化多酚氧化酶的研究 [J]. 林产化学与工业, 2000, 2 (20): 23-26.
[13] 罗贵民. 酶工程 [M] . 北京:化学工业出版社, 2003 : 26-27.
[14] S. Vishwanath, D. Bhattacharyya, W. Huang, et al. Site-directed and random enzyme immobilization on functionalized membranes: kinetic studies and models[J]. Journal of Membrane Science, 1995, 108 (2): 1-13.

Service：

【Download】【Collect】

《膜科学与技术》编辑部 Address: Bluestar building, 19 east beisanhuan road, chaoyang district, Beijing; 100029 Postal code; Telephone：010-80492417/010-80485372; Fax：010-80485372 ; Email：mkxyjs@163.com