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New membrane material containing macrocyclic compounds for affinity adsorption of biomolecules

Authors： WANG Andong , LIN Ligang , DONG Meimei , SUN Hui , GAO Changchen，ZHANG Yuzhong

Units： School of Materials Science and Engineering , State Key Laboratory of Hollow Fiber Membrane Materials and Processes of Ministry of Education ,

KeyWords： macrocyclic compounds;affinity membrane;adsorption

ClassificationCode：TQ028.8

year,volume(issue):pagination： 2014,34(3):53-58

Abstract：

Using polyether sulfone(PES) and ethylene-vinyl alcohol copolymer(EVAL) as membrane matrix material, cyclodextrin macrocyclic compounds was adopted as functive, the reactive dyes ligands were coupled to the basement membrane by making use of the rich active groups on the functive, and a new type of affinity membrane containing cyclodextrin was prepared. The effects of the CD content and, the pH of BSA buffer solutions, ionic strength, solution concentration and adsorption time were investigated. It showed that the optimum adsorption conditions are; pH:5.0, cyclodextrin content:10%, ionic strength:0, solution concentration:1.0g/L and adsorption time:4h, adsorption capacity reached 57.7mg/g under the optimum adsorption conditions. The unique cavity structure and properties of cyclodextrins(CD) with hydrophilic external and hydrophobic internal cavities are presented to induct and pack biological macromolecules such as protein. The existence of cyclodextrin macrocyclic compounds benefits to the performance of the membrane adsorption. The adsorption capacity was enhanced with the increase of CD content in the membrane adsorbents within limits.

Funds：

国家自然科学基金(21006070)、天津市应用基础及前沿技术研究计划重点项目(11JCZDJC23700、11JCZDJC21200)、教育部科学技术项目、天津科技创新体系及平台建设计划

AuthorIntro：

王安栋（1986-），男，山东潍坊市人，天津工业大学在读研究生，师从林立刚副教授，主要从事吸附材料和膜技术在清洁生产中应用研究。*通讯联系人，E-mail：phdlinligang@163.com

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