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Separation and purification of Poly(β-L-malic acid) in fermentation broth of Aureobasidium pullulans by a two-step ultrafiltration

Authors： Bi Yicheng, Wang Hao, Cheng Ruodong, Zhou Hua*, Wei Ping

Units： College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211316, P. R. China.

KeyWords： Poly(β-)malic acid; two-step ultrafiltration; separation;

ClassificationCode：TQ028.8

year,volume(issue):pagination： 2015,35(1):97-102

Abstract：

In this study, a two-step ultrafiltration was applied to separate and concentrate PMLA from fermentation broth. Membranes with MWCO of 30 kDa and 1 kDa were feasible for separating PMLA from Pullulan and concentration, respectively. The best operational conditions of 30kDa membrane were as follows: transmembrane pressure 0.075MPa, dilution factor of 4, volume reduction ratio of 8, times of dilution diafiltration 1. The concentration factor of 1 kDa membrane was 4. At the optimal conditions, the purity of PMLA increased to 82.3%, and the recovery rate was 57.56%.

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AuthorIntro：

毕艺成（1990-），女，安徽省怀宁人，硕士研究生，主要研究方向为生物高分子的提取与应用。

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