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Preparation of PVDF plasma separation adsorption membrane by modified β-cyclodextrin for bilirubin removal

Authors： NI Feifei1, SHU Guiming 2, LI Ke 4, LU Xiaolong 1*, LIU Juanjuan 1,2,3, LI Tao 2,3

Units： 1.State Key Laboratory of Separation Membranes and Membrane Processes, School of Material Science and Engineering, Institute of Biological and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, PR China; 2. Tianjin Key Laboratory of Artificial Cell, Tianjin Institute of Hepatobiliary Disease, Tianjin Third Central Hospital, Tianjin 300170, PR China; 3.Tianjin Enterprises Key Laboratory of Blood Perfusion Technology, Tianjin ZiBo High technology Co.LTD, Tianjin 300170, PR China; 4.Tianjin Children’s Hospital, Tianjin 300074, PR China.

KeyWords： β-cyclodextrin; bilirubin adsorption; PVDF membrane; blood purification ; membrane adsorption; blood compatibility

ClassificationCode：TQ316.6+5

year,volume(issue):pagination： 2018,38(3):16-24

Abstract：

Due to the traditional filtration adsorption is achieved in two components with long process and high risk of infection. Therefore, a one-step filtration adsorption membrane was prepared, allowing filtration and adsorption to be completed in one module. PVDF hollow plasma membrane was used as the matrix material. Mono-substituted β-CD was used as adsorption ligand for bilirubin. Therefore a new type of PVDF plasma separation adsorption membrane was prepared for one-step filtration adsorption. So the chemical composition of the modified β-cyclodextrin and the membrane was characterized by infrared spectroscopy and nuclear magnetic resonance spectroscopy. The bubble point test and flux test of the membrane were used to characterize the surface and cross-sectional morphologies and scanning electron microscopy and atomic force microscopy was used to investigate separation performance of the membrane. Finally, the adsorption ability of modified membrane on BSA-bound bilirubin was tested by mimic plasma The results showed that β-CD was successfully grafted on the permeation side of PVDF plasma membrane. The size of modified membrane, the maximum pore size was 0.480μm, was almost unchanged compared with that of plasma separation membrane. Therefore the blood cells won’t permeate through modified membrane and plasma and small molecule toxins can permeate. Adsorption rate of 41.2% in one-step filtration adsorption was basically the same as direct adsorption (43.5%).

Funds：

天津市科技计划重点项目（15ZCZDGX00280）；天津市企业重点实验室项目（16PTSYJC00210）；天津市科技支撑项目（12ZCZDSY02700）

AuthorIntro：

倪非非（1993-），男，安徽芜湖市人，硕士研究生，从事PVDF膜表面改性及胆红素吸附应用研究；E-mail：1520406641@qq.com。 *通讯作者，E-mail：luxiaolong@263.net；E-mail：litao0387@sohu.com

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