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Mechanism of Escherichia coli inactivation by electrocatalytic membrane

Authors： WANG Yin, ZHAO Lei, DENG Cheng, LI Shuangrong, CHEN Xiao, LIU Hongbin, ZHU Mengfu

Units： Institute of Medical Support Technology, Academy of System Engineering, Academy of Military Science of Chinese People’s Liberation Army, Tianjin 300161, China

KeyWords： electrocatalytic membrane; E.coli; inactivation; oxidation; damage mechanism

ClassificationCode：TQ028.8

year,volume(issue):pagination： 2022,42(2):34-39

Abstract：

In this study, an electrocatalytic membrane was used to inactivate E. coli and the mechanism was identified through a series of technical methods. The EPR measurement was used to detect free radicals produced during the electrocatalysis process. The plate counting, electron microscope observation，dual staining，resazurin reduction test and other technical methods were used to determine the cultivability, morphological structure, cell membrane permeability and metabolic capacity of Escherichia coli. These results showed that the electrocatalytic membrane can produce reactive oxygen species such as hydroxyl radicals and singlet oxygen during the treatment process, and can destroy the structure, bacterial reductase and metabolism of E.coli, thus endowing it the ability of inactivating bacteria. The effect of the electrocatalytic membrane for inactivating E. coli increased with the increase of current density. When the current density reached 75 mA·cm-2, the total number of E. coli colonies decreased by 4.1 orders of magnitude, and the percentage of living bacteria with metabolic ability decreased to 32.4%.

Funds：

国家自然科学基金项目（51878659）；军队后勤科研重大项目（AWS18J004）

AuthorIntro：

王尹（1997-），男，江苏苏州人，硕士生，研究方向为膜分离净化技术，E-mail：1224582874@qq.com

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