膜科学与技术

Position：Home >> Abstract

Investigation on separation of residual sugar and lactic acid from straw fermentation broth by nanofiltration technology

Authors： GUO Pei, ZHAO Liming, LIU Lujie, QIU Yongjun, LI Shuang

Units： 1 State Key Laboratory of Bioreactor Engineering, Research and Development Centre of Separation and Extraction Technology in Fermentation Industry, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China;2 Shanghai Guanshengyuan Food Co., Ltd., Shanghai 200237, China

KeyWords： L-lactic acid; residual sugar; straw fermentation broth; nanofiltration; CFSK model

ClassificationCode：TQ028. 8

year,volume(issue):pagination： 2023,43(3):104-115

Abstract：

This study aims to investigate the nanofiltration membrane separation technology to separate the residual sugars and the L-lactic acid from straw biomass fermentation broth. Based on the unique multi-component fermentation broth system with high concentration of L-lactic acid, the effects of pH, transmembrane pressure, temperature and ionic strength on the separation of residual sugars and L-lactic acid were investigated. Based on the optimal nanofiltration conditions (pH=2.00, 2 MPa, 25 ºC), a three-stage nanofiltration separation process was designed to perform nanofiltration separation on the actual fermentation broth. The purity (wt/wt %) of L-lactic acid was increased from 92.4% to 99.7%. The yield of L-lactic acid was higher than 80.0% in each stage, and the ideal effect of decolorization were achieved simultaneously. In terms of mechanism, the applicability and accuracy of CFSK model in describing nanofiltration separation of lactic acid and residual sugar in the straw fermentation broth system were established for the first time by comparing the internal and external interpolation results of the model with the experimental data.

Funds：

国家重点研发计划项目（2017YFB0309302）；国家自然科学基金项目（31371725）；上海市曙光计划项目（15SG28）。

AuthorIntro：

郭佩（1994-），女，湖北黄冈人，硕士，从事纳滤膜分离研究，E-mail：18616011302@163.com。

Reference：

[1] Es I, Mousavi Khaneghah A, Barba F J, et al. Recent advancements in lactic acid production − a review[J]. Food Research International, 2018, 107: 763-770.
[2] Ramot Y, Haim-Zada M, Domb A J, et al. Biocompatibility and safety of PLA and its copolymers[J]. Advanced Drug Delivery Reviews, 2016, 107: 153-162.
[3] Jem K J, Tan B. The development and challenges of poly (lactic acid) and poly (glycolic acid) [J]. Advanced Industrial and Engineering Polymer Research, 2020, 3(2): 60-70.
[4] Lv X, Guo Y, Zhuang Y, et al. Optimization and validation of an extraction method and HPAEC-PAD for determination of residual sugar composition in L-lactic acid industrial fermentation broth with a high salt content[J]. Analytical Methods, 2015, 7(21): 9076-9083.
[5] Komesu A, Maciel M R W, de Oliveira R A, et al. Influence of residual sugars on the purification of lactic acid using short path evaporation[J]. Bioresources, 2017, 12(2): 4352-4363.
[6] Oliveira R A d, Komesu A, Vaz Rossell C E, et al. A study of the residual fermentation sugars influence on an alternative downstream process for first and second-generation lactic acid[J]. Sustainable Chemistry and Pharmacy, 2020, 15: 100206.
[7] Pinelo M, Jonsson G, Meyer A S. Membrane technology for purification of enzymatically produced oligosaccharides: molecular and operational features affecting performance[J]. Sep Purif Technol, 2009, 70(1): 1-11.
[8] Dey P, Pal P. Direct production of L (+) lactic acid in a continuous and fully membrane-integrated hybrid reactor system under non-neutralizing conditions[J]. Journal of Membrane Science, 2012, 389(none):355-362.
[9] Oonkhanond B, Jonglertjunya W, Srimarut N, et al. Lactic acid production from sugarcane bagasse by an integrated system of lignocellulose fractionation, saccharification and fermentation, and ex-situ nanofiltration[J]. Journal of Environmental Chemical Engineering, 2017, 5(3):2533-2541.
[10] Ma K, Hu G, Pan L, et al. Highly efficient production of optically pure L-lactic acid from corn stover hydrolysate by thermophilic Bacillus coagulans[J]. Bioresource Technology, 2016, 219: 114-122.
[11] Qiu Z, Gao Q, Bao J. Engineering pediococcus acidilactici with xylose assimilation pathway for high titer cellulosic L-lactic acid fermentation[J]. Bioresource Technology, 2017, 249: 9-15.
[12] Liu G, Sun J, Zhang J, et al. High titer l-lactic acid production from corn stover with minimum wastewater generation and techno-economic evaluation based on Aspen plus modeling[J]. Bioresource Technology, 2015, 198:803-810.
[13] 赵凯, 许鹏举, 谷广烨. 3,5-二硝基水杨酸比色法测定还原糖含量的研究[J]. 食品科学, 2008, 29(8): 534-536.
[14] 赵黎明. 膜分离技术在食品发酵工业中的应用[M]. 中国纺织出版社, 2011.
[15] Spiegler K S, Kedem O. Thermodynamics of hyperfiltration (reverse osmosis): Criteria for efficient membranes [J]. Desalination, 1966, 1(4): 311-326.
[16] Murthy Z V P, Chaudhari L B. Rejection behavior of nickel ions from synthetic wastewater containing Na2SO4, NiSO4, MgCl2 and CaCl2 salts by nanofiltration and characterization of the membrane[J]. Desalination, 2009, 247(1-3): 610-622.
[17] Zhu J, Guo N, Zhang Y, et al. Preparation and characterization of negatively charged PES nanofiltration membrane by blending with halloysite nanotubes grafted with poly (sodium 4-styrenesulfonate) via surface-initiated ATRP[J]. Journal of Membrane Science, 2014, 465:91-99.
[18] Luo J, Wan Y. Effects of pH and salt on nanofiltration-a critical review[J]. Journal of Membrane Science, 2013, 438:18-28.
[19] Kunz W, Henle J, Ninham B W. ‘Zur Lehre von der Wirkung der Salze’ (About the science of the effect of salts): Franz Hofmeister's historical papers[J]. Current Opinion in Colloid and Interface Science, 2004, 9(1/2): 19-37.
[20] Freger V, Arnot T C, Howell J A. Separation of concentrated organic/inorganic salt mixtures by nanofiltration[J]. Journal of Membrane Science, 2000, 178(1): 185-193.

Service：

【Download】【Collect】

《膜科学与技术》编辑部 Address: Bluestar building, 19 east beisanhuan road, chaoyang district, Beijing; 100029 Postal code; Telephone：010-80492417/010-80485372; Fax：010-80485372 ; Email：mkxyjs@163.com