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Preparation and adsorption properties of CTA hollow fiber affinity chromatography

Authors： LI Shuqin, WU Jiaojie, ZHOU Junjie, WEI Yongming, XU Zhenliang, YANG Hu

Units： Joint State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Research Center of Functional Membrane Science and Engineering, Shanghai 200237，China

KeyWords： surface modification; CTA hollow fiber affinity chromatography; static adsorption; dynamic adsorption

ClassificationCode：TQ028.3

year,volume(issue):pagination： 2024,44(2):97-106

Abstract：

In order to explore the adsorption properties of cellulose triacetate (CTA) hollow fiber affinity chromatography, the CTA hollow fiber membrane was used as the basal membrane, The surface of the membrane was modified by hydrolysis, crosslinking activation of 1,4-butanediol diglycidyl ether (EGDE), grafting 1,6-hexanediamine (HMDA) as the spacer arm, and then the modified CTA hollow fiber affinity chromatography was prepared by chelating Cu2+ with iminodiacetic acid (IDA) as ligand. In the static adsorption study of γ-globulin: when the concentration of γ-globulin adsorption solution is 1 mg/mL, ion concentration is 0.2mol/L and pH=8, the maximum static adsorption capacity is 2.4mg/cm3. The adsorption process accords with Langmuir isothermal adsorption model. For the study of dynamic adsorption of γ-globulins: within the scope of experimental studies, the penetration curves at different flow rates were similar to "S" shape, and the flow rate did not affect the binding ability of γ-globulin on CTA hollow fiber affinity chromatography; The dynamic adsorption capacity of γ-globulin increased with the increase of initial concentration.

Funds：

国家自然科学基金项目(21978082，22278132，22078092).

AuthorIntro：

李淑琴（1997-），女，湖北仙桃市人，硕士生，研究方向为分离膜的制备及应用研究，E-mail: lsq13677292559@163.com

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