膜科学与技术

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Preparation of PVDF chromatographic membrane and its application to colloidal gold test paper

Authors： BIAN Qingdong1,2， LI Yonghui1,2， WANG Zhaohui1,2， ZHOU Yue1,2， WANG Xiaozu1,2， CUI Zhaoliang1,2

Units： 1．State Key Laboratory of Materials-Oriented Chemical Engineering， College of Chemical Engineering，Nanjing Tech University， Nanjing 211800，China； 2．National Engineering Research Center for Special Separation Membrane， Nanjing Tech University， Nanjing 211800， China

KeyWords： PVDF; vapour-induced phase separation method; surface coating modification; lateral flow immunochromatography

ClassificationCode：TQ028.8

year,volume(issue):pagination： 2025,45(1):70-77

Abstract：

Nitrocellulose (NC) membrane is commonly used in immunochromatography, but it is monopolised by foreign countries and is flammable and unstable. Polyvinylidene fluoride (PVDF) has the advantages of good chemical stability and strong protein adsorption. PVDF as the membrane material, dimethylacetamide (DMAc) as the solvent, polyvinylpyrrolidone (PVPK90) and polyethylene glycol (PEG600) as additives, the membranes were prepared by the vapour-induced phase separation method. The influences of additive PEG600 content, vapour induction time and vapour temperature on membranes structure, pore size, contact angle, and protein adsorption were investigated; PVDF membranes were modified using different types of surfactants, and the membranes were tested by dynamic contact angle, capillary flow time, and protein adsorption which were then prepared as test strips. The results showed that when the membranes was made under the conditions of 2% PEG600, steam induction time of 20 min and steam temperature of 50 ℃, the pore size of the membranes was about 7.4 μm, the contact angle was 85.1°, and the protein adsorption amount was 22.6 μg/cm2, the capillary flow time of the PVDF membranes modified with sodium methylcocoyl taurate (CMT) was 199 s, and the protein adsorption amount reached 75.7 μg /cm2, and the test strips had the ability to develop colour.

Funds：

国家重点研发计划项目（2023YFB3810502）；国家自然科学基金项目（22078146）

AuthorIntro：

卞庆东（2001-），男，江苏徐州人，硕士生，从事膜材料研究

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