膜科学与技术

位置：首页 >> 期刊内容阅读

乙烯-乙烯醇共聚物智能开关膜的pH响应性与蛋白分离性能研究

作者：杨彬¹，²，叶卉¹，²，刘琦¹，²，李安妮¹，²，张玉忠¹，²，赵莉芝¹，²，李泓¹，²

单位： 1.天津工业大学材料科学与工程学院，天津300387； 2. 省部共建分离膜与膜过程国家重点实验室，天津工业大学，天津300387

关键词：智能开关膜；pH响应性；乙烯-乙烯醇共聚物；蛋白分离

DOI号：

分类号： TQ028.8

出版年,卷(期):页码： 2016,36(3):48-53

摘要：

采用膜技术对蛋白质进行分离纯化已受到生命科学领域的广泛关注，而智能开关膜为蛋白质精细分离提供了新的解决方案。本文以接枝甲基丙烯酸-N,N-二甲氨基乙酯（DMAEMA）的乙烯-乙烯醇共聚物（EVAL）分离膜为智能开关膜，对其开关特性与蛋白分离性能进行了研究。结果表明，EVAL接枝膜渗透通量具有显著的开关性，且pH交替重复6个单元以后开关性能保持稳定。在模拟蛋白分子牛血清白蛋白（BSA）与溶菌酶（LZM）及混合模拟蛋白分子BSA与LZM分离过程中，接枝率为15%的EVAL接枝膜在pH 3.6的条件下对BSA与LZM分离效果最佳，分离系数分别为9.7和7.8。

Membrane technique used for protein separation has gained great attention in the life science field.Intelligent switch membranes provide an efficient method for efficient protein separation. In this research, intelligent switch ethylene vinyl alcohol copolymer (EVAL) membranes were prepared by grafting dimethylaminoethylmethacrylate(DMAEMA) using UV irradiation graft polymerization.ThepH–responsive and protein separation properties of the intelligent switch membranes were investigated. The results indicated that the pH-responsive “on/off” properties of the grafted EVAL membrane are observed. During six cyclic measurements, the pH-responsive “on/off” properties are also observed. During the separation of bovine serum albumin (BSA) / lysozyme (LZM) model solution andBSA/LZM binary mixture, the highest separation factoris achieved at pH 3.6 of poly(DMAEMA)-grafted EVAL membrane with GD 15%.The separation factor values are 9.7 and 7.8, respectively.

基金项目：

国家自然科学基金资助项目（51373120,51173132,21204064）；高等学校博士学科点专项科研基金（20111201110003）；教育部团队；中空纤维膜材料与膜过程省部共建国家重点实验室培育基地开放课题；天津市大学生创新创业训练计划项目（201410058082）

作者简介：

第一作者：杨彬（1989—），男，硕士研究生，天津人，主要研究方向为功能膜分离。通信作者： E-mail：qianye325326@163.com

参考文献：

[1] Yao CH, Chuang WY, Chen YS. In vitro study of the effect of doxorubicin released from
EVAL membrane on vesical cancer cells[J].Material chemistry and physics.2002,73(1):1-5.
[2] 叶卉. 乙烯-乙烯醇共聚物分离膜制备及其乳清资源化利用研究 [D]. 天津: 天津工业
大学, 2012.
[3] Hong KH, Liu N, Sun G. UV-induced graft polymerization of acrylamide on cellulose by
using immobilized benzophenone as a photo-initiator[J].European Polymer Journal.2009,45(8):2443-2449.
[4] 白丽燕, 陈春琴, 林东强. 蛋白质水力学半径的测定及用于蛋白质变性过程的实时监控
[J].化工学报.2008,59(6):1486-1489.
[5] A. Bonincontro, A De Francesco, Onori G. Influence of pH on lysozyme conformation
revealed by dielectric spectroscopy[J].Colloids and Surfaces B: Biosurfaces.1998(1),12:1-5.
[6] Li Q, Bi Q Y, Lin H H. A novel ultrafiltration (UF) membrane with controllable selectivity
for protein separation[J].Journal of Membrane Science.2013,427(15):155-167.
[7] Zhao C S, Nie S Q, Tang M. Polymeric pH-sensitive membranes—A review[J].
Progress in Polymer Science.2011,36(11):1499-1520.
[8] Zhu M N, Xiong L J, Wang T. High tensibility and pH-responsive swelling of nanocomposite
hydrogels containing the positively chargeable 2-(dimethylamino)ethyl methacrylate monomer[J].Reactive and Functional Polymers.2010,70(5):267-271.
[9] 杜军, 何霞, 唐金晶. pH 值敏感响应膜的制备和性能研究[J].材料导报.2007,21(8):369-372.
[10]Xu FJ, Kang ET, Neoh KG. pH- and temperature-responsive hydrogels from crosslinked
triblock copolymers prepared via consecutive atom transfer radical polymerizations[J].Biomaterials.2006,27(14):2787-2797.
[11] Chakrabarty T, Shahi V. (3-glycidoxypropyl) Trimethoxy silane induced switchable
zwitterionic membrane with high protein capture and separation properties[J].Journal of Membrane Science.2013,444(1):77-86.
[12] Kumar M, Ulbricht M. Novel ultrafiltration membranes with adjustable charge density based on sulfonated poly(arylene ether sulfone) block copolymers and their tunable protein separation performance[J].Polymer.2014,55(1):354-365.

服务与反馈：

【文章下载】【加入收藏】

《膜科学与技术》编辑部地址：北京市朝阳区北三环东路19号蓝星大厦邮政编码：100029 电话：010-64426130/64433466 传真：010-80485372邮箱：mkxyjs@163.com