膜科学与技术

用于胆红素吸附的β-环糊精改性PVDF血浆分离膜的制备

作者：倪非非 ¹，舒桂明²，李可⁴，吕晓龙¹，刘娟娟¹，²，³，李涛²、³

单位： 1分离膜与膜过程国家重点实验室，材料科学与工程学院，生物化工研究所，天津工业大学，天津 300387；2卫生部人工细胞工程技术研究中心，天津市第三中心医院，天津，300170；3天津市血液灌流技术企业重点实验室，天津市紫波高科技有限公司，天津，300170；4天津市儿童医院

关键词： β-环糊精；胆红素；PVDF膜；血液净化；膜吸附；血液相容性

DOI号：

分类号： TQ316.6+5

出版年,卷(期):页码： 2018,38(3):16-24

摘要：

由于传统滤过吸附在两个组件中实现，流程长，感染风险大。因此，制备了一种一步滤过吸附用膜，使滤过吸附在一个组件中完成。本文用PVDF中空血浆分离膜作为基体材料，单取代改性β-环糊精（β-CD）作为胆红素的吸附配基，仅在膜透过侧接枝β-CD，制备了一种新型PVDF血浆分离吸附膜。实验中采用红外光谱、核磁测试等测试手段对改性β-CD和膜的化学成分进行表征分析。用扫描电镜、原子力显微镜等方法验证了改性膜的表面结构及断面结构，泡点测试和通量测试去表征改性膜的分离性能。最后通过模拟血浆测试了改性膜对BSA结合胆红素的吸附能力。结果表明，β-CD成功接枝到的PVDF血浆分离膜的透过侧；改性膜相比血浆分离膜膜孔大小基本不变，最大孔径为0.480μm，满足细胞不能透过，血浆和胆红素能透过的要求；一步滤过吸附方式的吸附率达到41.2%，与直接吸附（43.5%）的效果基本一致。

Due to the traditional filtration adsorption is achieved in two components with long process and high risk of infection. Therefore, a one-step filtration adsorption membrane was prepared, allowing filtration and adsorption to be completed in one module. PVDF hollow plasma membrane was used as the matrix material. Mono-substituted β-CD was used as adsorption ligand for bilirubin. Therefore a new type of PVDF plasma separation adsorption membrane was prepared for one-step filtration adsorption. So the chemical composition of the modified β-cyclodextrin and the membrane was characterized by infrared spectroscopy and nuclear magnetic resonance spectroscopy. The bubble point test and flux test of the membrane were used to characterize the surface and cross-sectional morphologies and scanning electron microscopy and atomic force microscopy was used to investigate separation performance of the membrane. Finally, the adsorption ability of modified membrane on BSA-bound bilirubin was tested by mimic plasma The results showed that β-CD was successfully grafted on the permeation side of PVDF plasma membrane. The size of modified membrane, the maximum pore size was 0.480μm, was almost unchanged compared with that of plasma separation membrane. Therefore the blood cells won’t permeate through modified membrane and plasma and small molecule toxins can permeate. Adsorption rate of 41.2% in one-step filtration adsorption was basically the same as direct adsorption (43.5%).

基金项目：

天津市科技计划重点项目（15ZCZDGX00280）；天津市企业重点实验室项目（16PTSYJC00210）；天津市科技支撑项目（12ZCZDSY02700）

作者简介：

倪非非（1993-），男，安徽芜湖市人，硕士研究生，从事PVDF膜表面改性及胆红素吸附应用研究；E-mail：1520406641@qq.com。 *通讯作者，E-mail：luxiaolong@263.net；E-mail：litao0387@sohu.com

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