膜科学与技术

结合胆红素特异性吸附PVC改性微孔膜制备研究

作者：杨悦，刘娟娟，吕晓龙，马荣花，郑书云，舒桂明，李可

单位： 1省部共建分离膜与膜过程国家重点实验室，材料科学与工程学院，生物化工研究所，天津工业大学，天津 300387； 2卫生部人工细胞工程技术研究中心，天津市第三中心医院，天津 300170； 3天津市血液灌流技术企业重点实验室，天津市紫波高科技有限公司，天津 300170； 4天津市儿童医院，天津 300074

关键词： PVC；二乙烯三胺；胆红素；血液净化；表面改性

DOI号：

分类号： TQ028.8

出版年,卷(期):页码： 2022,42(5):86-93

摘要：

临床上主要采用血液灌流技术治疗高胆红素血症，但血液灌流树脂的血液相容性较差，且由于治疗过程中需要介入血浆分离器，增加了治疗风险和治疗成本。因此本文创新性的研制了一种具有一步“滤过-吸附”双功能的新型聚氯乙烯（PVC）改性膜，通过傅里叶红外光谱、X射线光电子能谱分析和扫描电镜等测试手段研究了膜的化学结构和形貌，测试原膜与改性膜的基本性能，并对原膜与改性膜的胆红素吸附能力，凝血性和溶血性进行了表征。结果表明，二乙烯三胺被成功接枝到膜表面，接枝改性后，改性膜的表面和断面结构未发生明显改变，最大分离孔径为0.27 μm，牛血清白蛋白筛分率达94%，纯水通量达445 L·m-2·h-1，胆红素吸附率达60%，溶血率小于5%，不会引起溶血反应，凝血时间和纤维蛋白原与空白对照组相比无明显变化，不会激起凝血反应。

The main clinical treatment for hyperbilirubinemia is hemoperfusion technique, but the blood compatibility of hemoperfusion resin is poor. The need of plasma separator during treatment increases the risk of treatment and the cost of treatment. Therefore, this paper creatively developed a new type of polyvinyl chloride (PVC) composite membrane with one-step "filtration adsorption" function. The chemical structure and morphology of the membrane were studied by means of Fourier infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy, the basic properties of the original membrane and composite membrane were tested, and the bilirubin adsorption capacity, coagulability and hemolysis of the original membrane and composite membrane were characterized. The results showed that diethylenetriamine was successfully grafted onto the membrane surface. After grafting modification, the surface and cross-section structure of the composite membrane did not change significantly, and the maximum separation pore size was 0.27 μm, the screening rate of bovine serum albumin was 94%, the pure water flux was 445 L·m-2·h-1, the adsorption rate of bilirubin was 60%. The hemolysis rate was less than 5%, which would not cause hemolysis reaction. There was no significant change in coagulation time and fibrinogen compared with the blank control group, and would not arouse coagulation reaction.

基金项目：

天津市高等学校新型膜材料及膜分离技术创新团队(No. TD13-5044)；天津市科技支撑项目(20YFZCSY00310)；天津市卫生健康科技项目(TJWJ2021MS014)；天津市重点实验室项目(16PTSYJC00210)

作者简介：

杨悦(1997?)，女，天津市，硕士生，研究方向为分离膜制备，E-mail:18222066890@163.com.

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