膜科学与技术

PVC/PVDF-CTFE共混中空纤维膜用于血液氧合的研究

作者：荣新雨¹，吕晓龙¹，²，刘娟娟³，谷杰¹，任凯¹

单位： 1. 天津工业大学省部共建分离膜与膜过程国家重点实验室，材料科学与工程学院，生物化工研究所，天津 300387； 2. 天津膜天膜科技股份有限公司膜材料与膜应用国家重点实验室, 天津 300334； 3. 天津市第三中心医院肝胆疾病研究所，天津 300170

关键词：人工肺；聚氯乙烯；中空纤维膜；非溶剂相转化法；血液相容性

DOI号： 10.16159/j.cnki.issn1007-8924.2024.06.007

分类号： TQ028.8

出版年,卷(期):页码： 2024,44(6):55-63

摘要：

氧合膜是体外膜氧合的核心材料，在平衡气体交换效率、耐血浆渗漏以及血液相容性方面仍存在许多问题.聚氯乙烯是一种常用的医用材料，本文提出将聚氯乙烯、聚偏氟乙烯-三氟氯乙烯材料用于制备氧合膜.采用非溶剂致相分离法制备疏水性中空纤维膜，然后通过戊二醛交联将羧甲基纤维素固定在中空纤维膜内表面，构建小孔径的氧合膜涂层.涂层一方面起到预过滤的作用，延长血浆渗漏时间；另一方面改善膜表面血液相容性.结果表明，构建涂层后，氧合膜孔径由375 nm减小至206 nm时，气体渗透通量为（661±58） mL/(cm2·min·MPa)，渗透通量仍高于聚4-甲基-1-戊烯膜，与商业聚丙烯（PP）膜相似.羧甲基纤维素涂层改善了膜表面血液相容性，BSA吸附率仅为0.9%，相比于商业PP膜，红细胞黏附量降低46.7%，血小板黏附量降低21.4%，溶血率仅为0.25%，是安全的生物膜材料.羧甲基纤维素涂层膜的渗漏时间延长至22 h，为商业PP膜的3.6倍.本研究制备的PVC/PVDF-CTFE氧合膜为新膜材料的开发和设计提供了一定的指导.

Oxygenation membrane is the core material for extracorporeal membrane oxygenation, and there are still many issues regarding the balance of gas exchange efficiency, plasma leakage resistance, and blood compatibility. Polyvinyl chloride (PVC) is a commonly used medical material. In this paper, polyvinyl chloride (PVC) and polyvinylidene fluoride-trifluorovinyl chloride (PVDF-CTFE) materials were first used to prepare oxygenated membranes. The hydrophobic hollow fiber membrane was prepared by non-solvent induced phase separation, and then carboxymethyl cellulose was fixed on the inner surface of hollow fiber membrane by glutaraldehyde crosslinking, and the oxygenated membrane coating with small pore size was constructed. On the one hand, the coating plays a pre-filtration role, prolonging the plasma leakage time, and on the other hand, improving the blood compatibility of the membrane surface. After coating construction, the pore size decreased from 375 nm to 206 nm, and the gas permeation flux was 661±58 mL/(cm2·min·MPa), higher than that of poly(4-methyl-1-pentene) membrane and similar to commercial polypropylene (PP) membrane. The carboxymethyl cellulose coating improved the blood compatibility of the membrane surface, with a BSA adsorption rate of only 0.9%. Compared with commercial PP membrane, the amount of red blood cell adhesion decreased by 46.7%, and the amount of platelet adhesion decreased by 21.4%. The hemolysis rate was only 0.25%, indicating it as a safe biomembrane material. The leakage time of carboxymethyl cellulose coating membrane was extended to 22 hours, which is 3.6 times longer than that of commercial PP membrane. The PVC/PVDF-CTFE oxygenated membrane prepared in this study provides certain guiding significance for the development and design of new membrane materials.

基金项目：

天津市科技计划重点项目(15ZCZDGX00280)；天津市高等学校新型膜材料及膜分离技术创新团队(TD13-5044)

作者简介：

荣新雨（1999-），女，山东德州人，硕士研究生，从事膜材料在生物医学领域的应用研究

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